+52 (55) 56232175 depto@bq.unam.mx

Departamento de Bioquímica

Dr. Oscar Flores Herrera

Laboratorio 3 6/4 (antes 4Bis), Edificio D, primer piso, Departamento de Bioquímica, Facultad de Medicina, UNAM
Tel. +52 (55) 56 23 25 10
Investigación de interés

 

BIOENERGETICA MITOCONDRIAL
Nuestro interés es sobre el estudio de la bioenergética mitocondrial en dos modelos: la placenta de humano, y el basidiomiceto Ustilago maydis. En los últimos años, nos hemos enfocado en el estudio de los supercomplejos de las mitocondrias de Ustilago maydis, particularmente los dímeros de la F1F0-ATP sintasa y los supercomplejos conformados por los complejos respiratorios I, III2 y IV. Se ha demostrado que los dímeros del complejo V participan en el plegamiento de las crastas mitocondriales. Respecto a los supercomplejos, su composición puede ser III2:IV, I:III2, o I:III2:IV siendo éstos últimos reconocidos como respirosomas, gracias a su capacidad de oxidar al NADH y reducir al oxígeno.
 

Hemos aislado estos supercomplejos y los hemos caracterizado cinéticamente. Ahora contamos con mutantes de las subunidades dimerizantes del complejo V y hemos iniciado el estudio de la repercusión de la eliminación de las subunidades dimerizantes en la estabilidad del dímero. Adicionalmente, estudiamos la arquitectura mitocondrial en las mutantes de las subunidades dimerizantes.

 
Líneas de Investigación
  • Bioenergética mitocondrial; supercomplejos respiratorios
Trayectoria profesional
  • Investigador Titular “A” TC, SNI: I
  • Facultad de Medicina, UNAM (desde 1991).
Formación académica
  • Doctorado en Investigación Biomédica Básica (2001), UNAM.
  • Maestría en Investigación Biomédica Básica (1996), UNAM.
  • Licenciatura en Biología, (1992), Facultad de Ciencias, UNAM.
Integrantes del laboratorio
  • Técnico Académico: Dra. Mercedes Esparza Perusquía
Estudiantes
  • Giovanni García Cruz (Estudiante de Licenciatura en Química, UNAM)
  • Italo Güinno Lorandi Camacho (Estudiante de Licenciatura en Biología, UNAM)
  • Stefanie Paola López Cervantes (estudiante de Licenciatura en Biología Experimental. UNAM-I)
  • Jaime Abraham de Lira Sánchez (Estudiante de Doctorado en Ciencias Biomedicas, UNAM)

Publicaciones

de Lira-Sánchez JA, Esparza-Perusquía M, Martínez F, Pardo JP and Flores-Herrera O. Heavy metals do not induce ROS production by mitochondrial respirasome. (2023) Biochim Biophys Acta Bioenerg. 1864, 148999 Pubmed Biochim Biophys Acta Bioenerg
Palacios-Luna JE, López-Marrufo MV, Bautista-Bautista G, Velarde-Guerra CS, Villeda-Gabriel G, Flores-Herrera O, Osorio-Caballero M, Aguilar-Carrasco JC, Palafox-Vargas ML, García-López G, Díaz-Ruíz O, Arechavaleta-Velasco F and Flores-Herrera H. Progesterone modulates extracellular heat-shock proteins and interlukin-1β in human choriodecidual after Escherichia coli infection. (2023) Placenta. 142, 85-94 Pubmed Placenta
Bolaños-Suárez V, Alfaro A, Espinosa AM, Medina-Martínez I, Juárez E, Villegas-Sepúlveda N, Gudiño-Zayas M, Gutiérrez-Castro A, Román-Bassaure E, Salinas-Nieves ME, Bruno-Muñoz S, Aranda C, Flores-Herrera O and Berumen J. The mRNA and protein levels of the glycolytic enzymes lactate dehydrogenase A (LDHA) and phosphofructokinase platelet (PFKP) are good predictors of survival time, recurrence, and risk of death in cervical cancer patients. (2023) Cancer Med. 12, 15632-15649 Pubmed Cancer Med PMC Article
Esparza-Perusquía M, Langner T, García-Cruz G, Feldbrügge M, Zavala G, Pardo JP, Martínez F and Flores-Herrera O. Deletion of the ATP20 gene in Ustilago maydis produces an unstable dimer of FF-ATP synthase associated with a decrease in mitochondrial ATP synthesis and a high HO production. (2023) Biochim Biophys Acta Bioenerg. 1864, 148950 Pubmed Biochim Biophys Acta Bioenerg
Pardo JP, Guerra-Sánchez G, Flores-Herrera O and Romero-Aguilar L. Isolation of Mitochondria from Protoplasts. (2022) Bio Protoc. 12, e4277 Pubmed Bio Protoc PMC Article
Lucero RA, Mercedes EP, Thorsten L, Giovanni GC, Michael F, Guadalupe Z, Pablo PJ, Federico M and Oscar FH. Deletion of the natural inhibitory protein Inh1 in Ustilago maydis has no effect on the dimeric state of the FF-ATP synthase but increases the ATPase activity and reduces the stability. (2021) Biochim Biophys Acta Bioenerg. 1862, 148429 Pubmed Biochim Biophys Acta Bioenerg
Ruiz-Gómez X, Vázquez-Pérez JA, Flores-Herrera O, Esparza-Perusquía M, Santiago-Olivares C, Gaona-Bernal J, Gómez B, Mejía-Nepomuceno F, Méndez C and Rivera-Toledo E. Steady-state persistence of respiratory syncytial virus in a macrophage-like cell line and sequence analysis of the persistent viral genome. (2021) Virus Res. 297, 198367 Pubmed Virus Res
Fuentes-Zacarias P, Arzate-Castañeda DA, Sosa-González I, Villeda-Gabriel G, Morales-Méndez I, Osorio-Caballero M, Helguera-Repetto AC, Díaz FN, García-López G, Flores-Herrera O, Arenas-Huertero F, Eslava-Campos C, Díaz-Ruíz O and Flores-Herrera H. Pseudomonas aeruginosa induces spatio-temporal secretion of IL-1β, TNFα, proMMP-9, and reduction of epithelial E-cadherin in human alveolar epithelial type II (A549) cells. (2021) Acta Biochim Pol. 68, 207-215 Pubmed Acta Biochim Pol
Matuz-Mares D, Flores-Herrera O, Guerra-Sánchez G, Romero-Aguilar L, Vázquez-Meza H, Matus-Ortega G, Martínez F and Pardo JP. Carbon and Nitrogen Sources Have No Impact on the Organization and Composition of Respiratory Supercomplexes. (2021) J Fungi (Basel). 7, Pubmed J Fungi (Basel) PMC Article
Romero-Aguilar L, Guerra-Sánchez G, Tenorio EP, Tapia-Rodriguez M, Matus-Ortega G, Flores-Herrera O, González J and Pardo JP. Rapamycin induces morphological and physiological changes without increase in lipid content in Ustilago maydis. (2020) Arch Microbiol. 202, 1211-1221 Pubmed Arch Microbiol
Urban-Sosa VA, Olvera-Sánchez S, Barrera D, Aragón-Hernández JP, Flores-Herrera O and Martínez F. Isocitrate dehydrogenase type 2 (IDH2) is part of a multiprotein complex for placental steroidogenesis. (2019) Placenta. 87, 30-37 Pubmed Placenta
Reyes-Galindo M, Suarez R, Esparza-Perusquía M, de Lira-Sánchez J, Pardo JP, Martínez F and Flores-Herrera O. Mitochondrial respirasome works as a single unit and the cross-talk between complexes I, III and IV stimulates NADH dehydrogenase activity. (2019) Biochim Biophys Acta Bioenerg. 1860, 618-627 Pubmed Biochim Biophys Acta Bioenerg
Ramírez-Camacho I, Flores-Herrera O and Zazueta C. The relevance of the supramolecular arrangements of the respiratory chain complexes in human diseases and aging. (2019) Mitochondrion. 47, 266-272 Pubmed Mitochondrion
Álvarez-Cabrera MC, Barrientos-Galeana E, Barrera-García A, Osorio-Caballero M, Acevedo JF, Flores-Herrera O, Díaz NF, Molina-Hernández A, García-López G and Flores-Herrera H. Secretion of heat shock -60, -70 kD protein, IL-1β and TNFα levels in serum of a term normal pregnancy and patients with pre-eclampsia development. (2018) J Cell Mol Med. 22, 5748-5752 Pubmed J Cell Mol Med PMC Article
Sosa-Gutiérrez JA, Valdéz-Solana MA, Forbes-Hernández TY, Avitia-Domínguez CI, Garcia-Vargas GG, Salas-Pacheco JM, Flores-Herrera O, Téllez-Valencia A, Battino M and Sierra-Campos E. Effects of Leaves Extract on High Glucose-Induced Metabolic Changes in HepG2 Cells. (2018) Biology (Basel). 7, Pubmed Biology (Basel) PMC Article
García-Gudiño I, Yllescas-Medrano E, Maida-Claros R, Soriano-Becerril D, Díaz NF, García-López G, Molina-Hernández A, Flores-Herrera O, Zavala-Díaz de la Serna FJ, Del Rosario Peralta-Pérez M and Flores-Herrera H. Microbiological comparison of blood culture and amplification of 16S rDNA methods in combination with DGGE for detection of neonatal sepsis in blood samples. (2018) Eur J Pediatr. 177, 85-93 Pubmed Eur J Pediatr
Esparza-Perusquía M, Olvera-Sánchez S, Pardo JP, Mendoza-Hernández G, Martínez F and Flores-Herrera O. Structural and kinetics characterization of the FF-ATP synthase dimer. New repercussion of monomer-monomer contact. (2017) Biochim Biophys Acta Bioenerg. 1858, 975-981 Pubmed Biochim Biophys Acta Bioenerg
Martinez F, Olvera-Sanchez S, Esparza-Perusquia M, Gomez-Chang E and Flores-Herrera O. Multiple functions of syncytiotrophoblast mitochondria. (2015) Steroids. 103, 11-22 Pubmed Steroids
Flores-Herrera O, Olvera-Sánchez S, Esparza-Perusquía M, Pardo JP, Rendón JL, Mendoza-Hernández G and Martínez F. Membrane potential regulates mitochondrial ATP-diphosphohydrolase activity but is not involved in progesterone biosynthesis in human syncytiotrophoblast cells. (2015) Biochim Biophys Acta. 1847, 143-152 Pubmed Biochim Biophys Acta
Esparza-Perusquía M, Olvera-Sánchez S, Flores-Herrera O, Flores-Herrera H, Guevara-Flores A, Pardo JP, Espinosa-García MT and Martínez F. Mitochondrial proteases act on STARD3 to activate progesterone synthesis in human syncytiotrophoblast. (2015) Biochim Biophys Acta. 1850, 107-17 Pubmed Biochim Biophys Acta
Robles-Martínez L, Guerra-Sánchez MG, Flores-Herrera O, Hernández-Lauzardo AN, Velázquez-Del Valle MG and Pardo JP. The mitochondrial respiratory chain of Rhizopus stolonifer (Ehrenb.:Fr.) Vuill. (2013) Arch Microbiol. 195, 51-61 Pubmed Arch Microbiol
Salmerón-Santiago KG, Pardo JP, Flores-Herrera O, Mendoza-Hernández G, Miranda-Arango M and Guerra-Sánchez G. Response to osmotic stress and temperature of the fungus Ustilago maydis. (2011) Arch Microbiol. 193, 701-9 Pubmed Arch Microbiol
Gomez-Concha C, Flores-Herrera O, Olvera-Sanchez S, Espinosa-Garcia MT and Martinez F. Progesterone synthesis by human placental mitochondria is sensitive to PKA inhibition by H89. (2011) Int J Biochem Cell Biol. 43, 1402-11 Pubmed Int J Biochem Cell Biol
Matus-Ortega MG, Salmerón-Santiago KG, Flores-Herrera O, Guerra-Sánchez G, Martínez F, Rendón JL and Pardo JP. The alternative NADH dehydrogenase is present in mitochondria of some animal taxa. (2011) Comp Biochem Physiol Part D Genomics Proteomics. 6, 256-63 Pubmed Comp Biochem Physiol Part D Genomics Proteomics
De los Rios Castillo D, Zarco-Zavala M, Olvera-Sanchez S, Pardo JP, Juarez O, Martinez F, Mendoza-Hernandez G, García-Trejo JJ and Flores-Herrera O. Atypical cristae morphology of human syncytiotrophoblast mitochondria: role for complex V. (2011) J Biol Chem. 286, 23911-9 Pubmed J Biol Chem PMC Article
Guevara-Flores A, Olvera-Sánchez S, Gómez-Concha C, Juárez O, Esparza-Perusquía M, Pardo JP, Mendoza-Hernández G, Martínez F and Flores-Herrera O. 5'-p-Fluorosulfonyl benzoyl adenosine inhibits an ecto-ATP-diphosphohydrolase in the tegument surface of Taenia crassiceps cysticerci. (2008) Mol Biochem Parasitol. 162, 123-33 Pubmed Mol Biochem Parasitol
Juárez O, Guerra G, Velázquez I, Flores-Herrera O, Rivera-Pérez RE and Pardo JP. The physiologic role of alternative oxidase in Ustilago maydis. (2006) FEBS J. 273, 4603-15 Pubmed FEBS J
Martínez F, Uribe A, Espinosa-García MT, Flores-Herrera O, García-Pérez C and Milán R. Calcium modulates the ATP and ADP hydrolysis in human placental mitochondria. (2002) Int J Biochem Cell Biol. 34, 992-1003 Pubmed Int J Biochem Cell Biol
Martínez F, Uribe A, Milán R, Teresa Espinosa-García M, Gracía-Pérez C and Flores-Herrera O. Differential effects of magnesium on the hydrolysis of ADP and ATP in human term placenta. Effect of substrates and potassium. (2002) Int J Biochem Cell Biol. 34, 1004-16 Pubmed Int J Biochem Cell Biol
López-Cervantes SP, Toledo-Pérez R, De Lira-Sánchez JA, García-Cruz G, Esparza-Perusquía M, Luna-López A, Pardo JP, Flores-Herrera O and Konigsberg M. Sedentary Lifestyles and a Hypercaloric Diets During Middle Age, are Binomial Conducive to Fatal Progression, That is Counteracted by the Hormetic Treatment of Exercise, Metformin, and Tert-Butyl Hydroquinone: An Analysis of Female Middle-Aged Rat Liver Mitochondria. (2024) Dose Response. 22, 15593258241272619 Pubmed Dose Response PMC Article
López-Cervantes SP, Sánchez NS, Calahorra M, Mena-Montes B, Pedraza-Vázquez G, Hernández-Álvarez D, Esparza-Perusquía M, Peña A, López-Díazguerrero NE, Alarcón-Aguilar A, Luna-López A, Flores-Herrera Ó and Königsberg M. Moderate exercise combined with metformin-treatment improves mitochondrial bioenergetics of the quadriceps muscle of old female Wistar rats. (2022) Arch Gerontol Geriatr. 102, 104717 Pubmed Arch Gerontol Geriatr
Rendón JL, Miranda-Leyva M, Guevara-Flores A, Martínez-González JJ, Del Arenal IP, Flores-Herrera O and Pardo JP. Insight into the Mechanistic Basis of the Hysteretic-Like Kinetic Behavior of Thioredoxin-Glutathione Reductase (TGR). (2018) Enzyme Res. 2018, 3215462 Pubmed Enzyme Res PMC Article
Guevara-Flores A, Del Arenal IP, Mendoza-Hernández G, Pardo JP, Flores-Herrera O and Rendón JL. Mitochondrial Thioredoxin-Glutathione Reductase from Larval Taenia crassiceps (Cysticerci). (2010) J Parasitol Res. 2010, Pubmed J Parasitol Res PMC Article
Alejandra Sánchez-Muñoz M, Valdez-Solana MA, Campos-Almazán MI, Flores-Herrera Ó, Esparza-Perusquía M, Olvera-Sánchez S, García-Arenas G, Avitia-Domínguez C, Téllez-Valencia A and Sierra-Campos E. Streptozotocin-Induced Adaptive Modification of Mitochondrial Supercomplexes in Liver of Wistar Rats and the Protective Effect of Lam. (2018) Biochem Res Int. 2018, 5681081 Pubmed Biochem Res Int PMC Article
Guevara-Flores A, Herrera-Juárez ÁM, Martínez-González JJ, Del Arenal Mena IP, Flores-Herrera Ó and Rendón JL. Differential expression of disulfide reductase enzymes in a free-living platyhelminth (Dugesia dorotocephala). (2017) PLoS One. 12, e0182499 Pubmed PLoS One PMC Article
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