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Dr. Guillermo Mendoza Hernández


+52 (55) 56232133

SNI II
PRIDE C
Doctor
Profesor de Tiempo Completo


Líneas de Investigación

Química y estructura de proteínas.

Estudio del proteoma en diferentes modelos por espectrometria de masas y/o secuencia amino-terminal.

Proyectos de Investigación

  • Estudio del proteoma en diferentes especies.

El objetivo central de este proyecto es purificar y caracterizar cinética y estructuralmente diversas proteínas oligoméricas. Actualmente estudiamos los procesos de disociación/reasociación, desnaturalización y replegamiento de proteínas en presencia de agentes desnaturalizantes.

Nuestros estudios aportan información sobre la estabilidad, estados de transición conformacional, intermediarios y mecanismo de plegamiento de proteínas oligoméricas. Identificación, purificación y caracterización de proteínas. Estudio del proteoma en diferentes modelos por espectrometria de masas y/o secuencia amino-terminal.

Publicaciones Relevantes

Publicaciones Recientes

  1. Rodríguez-Alvarez M, Mendoza-Hernández G, Encarnación S, Calva JJ, López-Vidal Y. Phenotypic differences between BCG vaccines at the proteome level. Tuberculosis (Edinb). 2009 Feb 19. [Epub ahead of print]
  2. González-Zamorano M, Mendoza-Hernández G, Xolalpa W, Parada C, Vallecillo AJ, Bigi F, Espitia C. Mycobacterium tuberculosis Glycoproteomics Based on ConA-Lectin Affinity Capture of Mannosylated Proteins. J Proteome Res. 2009 Feb 6;8(2):721-733.
  3. Tellez LA, Blancas-Mejia LM, Carrillo-Nava E, Mendoza-Hernández G, Cisneros DA, Fernández-Velasco DA. Thermal unfolding of triosephosphate isomerase from Entamoeba histolytica: dimer dissociation leads to extensive unfolding. Biochemistry. 2008; 47 (44):11665-73.
  4. Guevara-Flores A, Olvera-Sánchez S, Gómez-Concha C, Juárez O, Esparza-Perusquía M, Pardo JP, Mendoza-Hernández G, Martínez F, Flores-Herrera O. 5'-p-Fluorosulfonyl benzoyl adenosine inhibits an ecto-ATP-diphosphohydrolase in the tegument surface of Taenia crassiceps cysticerci. Mol Biochem Parasitol. 2008;162 (2):123-33.
  5. García LM, Contreras-Zentella ML, Jaramillo R, Benito-Mercadé MC, Mendoza-Hernández G, del Arenal IP, Membrillo-Hernández J, Escamilla JE. The succinate:menaquinone reductase of Bacillus cereus: characterization of the membrane-bound and purified enzyme. Can J Microbiol. 2008; 54(6):456-66.
  6. Cabrera N, Hernández-Alcántara G, Mendoza-Hernández G, Gómez-Puyou A, Perez-Montfort R. Key residues of loop 3 in the interaction with the interface residue at position 14 in triosephosphate isomerase from Trypanosoma brucei. Biochemistry. 2008 Mar 18;47(11):3499-506.
  7. Rendón JL, Del Arenal IP, Guevara-Flores A, Mendoza-Hernández G, Pardo JP. Glucose 6-phosphate dehydrogenase from larval Taenia crassiceps (cysticerci): purification and properties.Parasitol Res. 2008 May;102(6):1351-7.
  8. Robles-Flores M, Meléndez L, García W, Mendoza-Hernández G, Lam TT, Castañeda-Patlán C, González-Aguilar H. Posttranslational modifications on protein kinase c isozymes. Effects of epinephrine and phorbol esters. Biochim Biophys Acta. 2008 ; 1783(5):695-712.
  9. Xolalpa W, Vallecillo AJ, Lara M, Mendoza-Hernandez G, Comini M, Spallek R, Singh M, Espitia C. Identification of novel bacterial plasminogen-binding proteins in the human pathogen Mycobacterium tuberculosis.Proteomics. 2007; 7(18):3332-3341.
  10. Fuentes-Silva D, Mendoza-Hernandez G, Stojanoff V, Palomares LA, Zenteno E, Torres-Larios A, Rodriguez-Romero A. Crystallization and identification of the glycosylated moieties of two isoforms of the main allergen Hev b 2 and preliminary X-ray analysis of two polymorphs of isoform II. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 ;63:787-791.
  11. Kinetic modeling can describe in vivo glycolysis in Entamoeba histolytica Saavedra, E., Marín-Hernández, A., Encalada, R., Olivos, A., Mendoza-Hernández, G., Moreno-Sánchez, R. 2007 FEBS Journal 274 (18), pp. 4922-4940
  12. Martínez-Becerra, F., Silva, D.-A., Domínguez-Ramírez, L., Mendoza-Hernández, G., López-Vidal, Y., Soldevila, G., García-Zepeda, E.A. Analysis of the antimicrobial activities of a chemokine-derived peptide (CDAP-4) on Pseudomonas aeruginosa. Biochem Biophys Res Commun. 2007 Apr 6;355(2):352-8.
  13. New insights into the unique structure of the F0F 1-ATP synthase from the chlamydomonad algae Polytomella sp. and Chlamydomonas reinhardtii Van Lis, R., Mendoza-Hernández, G., Groth, G., Atteia, A. 2007 Plant Physiology 144 (2), 1190-1199
  14. Analysis of the antimicrobial activities of a chemokine-derived peptide (CDAP-4) on Pseudomonas aeruginosa 2007 Biochemical and Biophysical Research Communications 355 (2), pp. 352-358
  15. Disulfide Bridges in the Mesophilic Triosephosphate Isomerase from Giardia lamblia Are Related to Oligomerization and ActivityReyes-Vivas, H., Diaz, A., Peon, J., Mendoza-Hernandez, G., Hernandez-Alcantara, G., De la Mora-De la Mora, I., Enriquez-Flores, S., (...), Lopez-Velazquez, G. 2007 Journal of Molecular Biology 365 (3), pp. 752-763
  16. Phytochelatin-cadmium-sulfide high-molecular-mass complexes of Euglena gracilis Mendoza-Cózatl, D.G., Rodríguez-Zavala, J.S., Rodríguez-Enríquez, S., Mendoza-Hernandez, G., Briones-Gallardo, R., Moreno-Sánchez, R. 2006 FEBS Journal 273 (24), pp. 5703-5713
  17. Carbohydrate affinity chromatography indicates that arylsulfatase-A from capacitated boar sperm has mannose and N-acetylglucosamine/sialic acid residues Jiménez, I., Fierro, R., González-Márquez, H., Mendoza-Hernández, G., Romo, S., Betancourt, M. 2006 Archives of Andrology 52, 455-462
  18. Circular dichroism of pig and bovine lactadherins and their affinity for the pig zona pellucidaZayas-Pérez, H., Tello-Solís, S.R., González-Márquez, H., Bonilla-González, E., Mendoza-Hernández, G., Betancourt-Rule, M. 2005 Protein and Peptide Letters 12 (3), pp. 299-303
  19. A novel 8.7 kDa protease inhibitor from chan seeds (Hyptis suaveolens L.) inhibits proteases from the larger grain borer Prostephanus truncatus (Coleoptera: Bostrichidae) Aguirre, C., Valdés-Rodríguez, S., Mendoza-Hernández, G., Rojo-Domínguez, A., Blanco-Labra, A. 2004 Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology 138 (1), pp. 81-89
  20. Bifunctional aldehyde/alcohol dehydrogenase (ADHE) in chlorophyte algal mitochondriaAtteia, A., Van Lis, R., Mendoza-Hernández, G., Henze, K., Martin, W., Riveros-Rosas, H., González-Halphen, D. 2003 Plant Molecular Biology 53 (1-2), pp. 175-188
  21. Identification of novel mitochondrial protein components of Chlamydomonas reinhardtii. A proteomic approachVan Lis, R., Atteia, A., Mendoza-Hernández, G., González-Halphen, D. 2003 Plant Physiology 132 (1), pp. 318-330